F12K+10%FBS+0.05 mg/ml抗壞血酸+ 0.01 mg/ml胰島素+0.01 mg/ml轉(zhuǎn)鐵蛋白+10 ng / ml亞硒酸鈉+ 0.03 mg/ml(ECGS)+10 mM HEPES+10 mM TES+1%P/S 貼壁